Success story: Unleashing unprecedented resolution and contrast in confocal FLIM with the PDA-23 SPAD array

Lausanne, July 22, 2024 — In the ever-evolving landscape of microscopy, researchers are constantly seeking new tools to unlock deeper insights into cellular dynamics and molecular interactions. Pi Imaging, a leader in advanced photon detection solutions, has answered this call with the introduction of the groundbreaking PDA-23 array detector. Download the success story.

The PDA-23 seamlessly integrates with the renowned Luminosa microscope from PicoQuant, poised to revolutionize the world of confocal fluorescence lifetime imaging (FLIM). This powerful add-on combines enhanced spatial resolution and improved contrast with the functional imaging capabilities of FLIM, opening up a new frontier in microscopy.

The synergy between the PDA-23's resolution enhancement and FLIM's functional data offers researchers an unprecedented opportunity to delve deeper into the intricacies of cellular processes. By achieving up to 40% more spatial resolution without changing fluorophores, we're empowering scientists to explore the nanoscale world with unparalleled clarity and detail.

The PDA-23's remarkable performance is achieved through its seamless integration with the Luminosa microscope and PicoQuant's industry-leading MultiHarp 160 TCSPC module. This perfect compatibility delivers high data quality. A dedicated ISM-FLIM software workflow ensures streamlined, hassle-free operation, with a daily auto-alignment process that makes the PDA-23 a robust and reliable tool for any research setting.

"One of the key advantages of the PDA-23 is its ease of use and seamless integration," explains Dr. Michel Antolovic, CEO at Pi Imaging. "Researchers can focus on their experiments, confident that the system will deliver consistent, high-quality results day after day. The combination of our SPAD detectors and PicoQuant's TCSPC technology ensures unparalleled precision, with a timing resolution of 100 ps and best-in-class dark counts."

But the PDA-23's capabilities extend far beyond just enhanced resolution and contrast. The microscope`s flexible software design and open data format empower researchers to create and adapt new time-resolved methodologies tailored to their specific research goals. Backed by over 25 years of expertise in photon detection, the PDA-23 provides a customizable platform for innovation, allowing scientists to push the boundaries of what's possible in microscopy.

"The PDA-23 is more than just a high-performance detector," says Dr. Antolovic. "It's a transformative tool that empowers researchers to explore the unknown, to uncover new insights, and to drive scientific progress in ways we've yet to imagine. By putting the power of cutting-edge technology in the hands of the research community, we're opening up new frontiers in the study of cellular dynamics and molecular interactions."

As the scientific community continues to seek ever-greater resolution and functional information, the PDA-23 array detector from Pi Imaging stands as a beacon of innovation, poised to elevate microscopy to new heights. Discover the power of this transformative technology and unlock the full potential of your research, click here to download the full document.