Improve sensitivity, extract molecular lifetimes and reduce sample damage
Life science applications utilize advanced techniques to study biological processes and states. Two prominent techniques are microscopy and flow cytometry. Within microscopy, confocal microscopes are the most advanced instruments providing excellent spatial resolution and high contrast when observing molecules, proteins and cells. Flow cytometers analyze multiple properties of cells rapidly flowing through a fluidic system. Both techniques usually color cell structures with bright fluorescent dyes to label specific structures of interest or to increase the contrast.
Fluorescence lifetime imaging microscopy (FLIM) additionally measures fluorescence (time) decay to analyze molecular composition and interactions.
These techniques are sensitive to sample damage due to overillumination. Thus, a high-sensitivity detector like a single-photon avalanche diode (SPAD) is needed. These techniques also require a high speed system due to scanning (in confocal microscopy) or the rapid flow (in flow cytometry). FLIM, additionally, requires precise picosecond timing precision.
Benefits of SPAD technology
for this industry
Single photon sensitivity
Due to high sensitivity, our detectors offer unprecedented data quality and image sharpness.
Picosecond timing precision
Extract molecular lifetimes with time-correlated single-photon counting (TCSPC).
Low sample damage
Use increased sensitivity to reduce acquisition time, phototoxicity and sample bleaching.
Examples / Application results
Confocal microscopy
SPAD arrays solve the main confocal challenge, the fundamental trade-off between signal intensity and resolution.
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